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Fig. 2. The changes in spectral fluorescence and reflectance are shown for corn leaves (Experiment I) that were provided in eight N applications rates between 0 and 150% of the optimal (100%) levels (n = 16 per group). Differences among treatments were highly significant for the two spectral regions in the green and red/far-red (Regions 1 and 2; delineated as dotted rectangles): (A) fluorescence emissions (400800 nm); (B) reflectance (400800 nm); and (C) the first derivative of the red-edge reflectance spectra, between 675 and 775 nm. Similar responses were observed across all three experiments.