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Fig. 7. Principal component analysis (PCA) of the soil microbial community shifts in cultivated sites as affected by different poultry litter application rates for 2003 and 2004 samplings according to the abundance of fatty acid methyl ester (FAME) indicators of fungal, bacterial, and actinomycetes groups. The symbols indicate the different poultry litter application rates applied to the soils (open symbols = 0, 4.5, and 6.7; closed symbols = 9.0, 11.2, and 13.4 Mg ha1). The PCA provides a comparison of the fungal to bacterial (F:B) populations ratio among the soils. The soils located near the microbial group specified indicate they have higher relative abundance of such microbial group.