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Fig. 1. Transcript levels of S. hamata SHST1 in transgenic Indian mustard lines. Total RNA was isolated from 3-wk-old wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. (A) Ethidium bromide-stained gel showing total RNA loading. (B) Autoradiogram of RNA blot after hybridization with ³³P-labeled SHST1 cDNA.