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Fig. 3. Selected ion chromatogram of spiked flushed dairy manure wastewater (FDMW) showing the retention time of the BSTFA [N,O-bis(trimethylsilyl)fluoroacetamide] derivatives of (a) estrone, (b) 17 ${alpha}$ -estradiol, (c) 17ß-estradiol, the internal standard (d) d₁₂-pyrelene, and (e) estriol after spiking with 40 ng of each estrogen analyte.