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Fig. 3. Concentration and time effects on total copper concentration ([Cu]tot) in water extracts from soil systems. (A) [Cu]tot of soil systems spiked with different levels of Cu after incubation for 1 h (circles), 19 h (squares), 8 d (triangles), 32 d (crosses), and 220 d (diamonds). Fitted linear regression lines for each incubation period are shown. (B) Recovery of amended Cu in water extracts as a function of the incubation time and dose of 100 (squares), 200 (crosses), 400 (triangles), and 800 (diamonds) µmol Cu kg1 soil. Fitted logarithmic regression lines for each Cu amendment are shown. The data presented are from duplicate soil systems and each point represents a single soil system. One data point (200 µmol Cu kg1 soil, 8 d) and two data points (800 µmol kg1 soil, 220 d) are missing due to loss of samples.