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Published online 1 May 2008
Published in J Environ Qual 37:741-752 (2008)
DOI: 10.2134/jeq2007.0086
© 2008 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
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A Comparison of Phosphorus Speciation and Potential Bioavailability in Feed and Feces of Different Dairy Herds Using 31P Nuclear Magnetic Resonance Spectroscopy

R. W. McDowella,*, Z. Doub, J. D. Tothb, B. J. Cade-Menunc, P. J. A. Kleinmand, K. Soderd and L. Saporitod

a AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
b Section of Animal Production Systems, School of Veterinary Medicine, Univ. of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348
c Dep. of Geological and Environmental Science, Stanford Univ., Building 320, Room 118, Stanford, CA 94305-2115
d USDA-ARS, Pasture Systems and Watershed Management Research Unit, Curtin Road, University Park, PA 16802-3702


Figure 1
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  		Fig. 1. 31P-nuclear magnetic resonance spectra for farm system 4 of fecal samples extracted while wet with NaOH-EDTA, water, or dilute HCl. Spectra are expanded to show detailed peak assignments in the region from 8 to –6 ppm. Mono, orthophosphate monoesters; orthoP, orthophosphate; myo-IHP, myo-inositol hexakisphosphate; lipids, phospholipids; TA, teichoic acid; oth. diest., other diesters; pyroP, pyrophosphate; polyP End Grp, polyphosphate end group. See the text for more details on peak assignments in these regions. Note spectra of dilute HCl extracts were limited by the solubility of Ca-P when made to pH >12.5 for analysis.

 

Figure 2
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  		Fig. 2. 31P-nuclear magnetic resonance spectra for system 4. Spectra are shown for diet (grain [feed] and forage [pasture]) and feces extracted wet or dry by NaOH-EDTA, water, or dilute HCl. PyroP, pyrophosphate; polyp, polyphosphate. Note spectra of dilute HCl extracts were limited by the solubility of Ca-P when made to pH >12.5 for analysis.

 




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