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Application of Enzyme-Linked Immunosorbent Assay Analysis for Determination of Monensin in Environmental Samples

^a Dep. of Plant and Earth Science, Univ. of Wisconsin-River Falls, 410 S. 3rd St., River Falls, WI 54022
^b Res. and Dev., Metropolitan Water Reclamation District of Greater Chicago, 6001 West Pershing Rd., Cicero, IL 60804
^c Dep. of Soil, Water, and Climate, Univ. of Minnesota, 1991 Upper Buford Circle, St. Paul, MN 55108
^d Veterinary Population Medicine, Univ. of Minnesota, 1333 Gortner Ave., St. Paul, MN 55108

View larger version (21K): [in this window] [in a new window]   Fig. 1. Structures of monensin, factors, and major metabolites isolated from cattle manure (Donoho et al., 1978) (adapted from Kiehl et al., 1998).

View larger version (14K): [in this window] [in a new window]   Fig. 2. Standard curve for the monensin ELISA. Error bars represent standard deviation of the mean. The working range of the monensin ELISA kit was approximately 2 to 100 µg L–1.

View larger version (17K): [in this window] [in a new window]   Fig. 3. Relationship between spiked and measured ELISA monensin concentrations for the water matrices.