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Published online 1 March 2008
Published in J Environ Qual 37:494-500 (2008)
DOI: 10.2134/jeq2007.0134
© 2008 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Evaluation of Phosphorus Characterization in Broiler Ileal Digesta, Manure, and Litter Samples: 31P-NMR vs. HPLC

A. B. Leytema,*, P. Kwanyuenb, P. W. Plumsteadc, R. O. Maguired and J. Brakec

a USDA-ARS, Northwest Irrigation and Soils Research Lab., 3793 N. 3600 E., Kimberly, ID 83341-5076
b USDA-ARS, Soybean and Nitrogen Fixation Research Unit, 3127 Ligon St., Raleigh, NC 27607
c Dep. of Poultry Science, College of Agriculture and Life Sciences, North Carolina State Univ., Raleigh, NC 27695-7608
d Crop and Soil Environmental Sciences Dep., Virginia Tech, Blacksburg, VA 24061


Figure 1
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  		Fig. 1. A sample 31P nuclear magnetic resonance spectroscopy (NMR) spectra obtained from one of the manure samples used in the study.

 

Figure 2
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  		Fig. 2. A sample high performance liquid chromatography (HPLC) chromatograph showing the inositol ester peaks.

 

Figure 3
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  		Fig. 3. Comparison of phytate determined in (a) broiler litter, (b) broiler manure, and (c) ileal digesta using 31P nuclear magnetic resonance spectroscopy (NMR) vs. high performance liquid chromatography (HPLC) analysis.

 

Figure 4
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  		Fig. 4. Comparison of monoester phosphate determined in (a) broiler litter, (b) broiler manure, and (c) ileal digesta using 31P nuclear magnetic resonance spectroscopy (NMR) analysis vs. total inositol phosphate measured in the same samples with high performance liquid chromatography (HPLC) analysis.

 




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