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Published online 4 January 2008
Published in J Environ Qual 37:207-218 (2008)
DOI: 10.2134/jeq2007.0128
© 2008 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
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Environmental Risk Assessment of Genetically Engineered Herbicide-Tolerant Zoysia japonica

T. W. Baea, E. Vanjildorjb, S. Y. Songb, S. Nishiguchia, S. S. Yangc, I. J. Songa, T. Chandrasekhara, T. W. Kangd, J. I. Kimc, Y. J. Kohe, S. Y. Parkf, J. Leeg, Y.-E. Leeh, K. H. Ryui, K. Z. Riua, P.-S. Songa,* and H. Y. Leea

a Faculty of Biotechnology, Cheju National Univ., Jeju 690-756, Korea
b Dep. of Horticulture, Chungnam National Univ., Daejeon 305-764, Korea
c Dep. of Biotechnology (BK21 Program) and Kumho Life Science Lab., Chonnam National Univ., Gwangju 500-757, Korea
d Applied Radiological Science Research Inst., Cheju National Univ., Jeju 690-756, Korea
e School of Environmental and Agricultural Science, Sunchon National Univ., Sunchon 540-742, Korea
f Dep. of Clinical Pathology, Cheju Halla College, Jeju 690-708, Korea
g School of Medicine, Cheju National Univ., Jeju 690-756, Korea
h Dep. of Biotechnology, Dongguk Univ., Kyungju, Kyongbuk 780-714, Korea
i Division of Environmental and Life Sciences, Seoul Women's Univ., Seoul 139-774, Korea


Figure 1
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Fig. 1. Field testing (a) and schematic illustration (b) for cross-hybridization between genetically modified (GM) and wild-type (WT) Zoysia grass at 0.5-m separation. Grass lanes, GM Zoysia grass; pots (25- cm diameter each), WT Zoysia grass.

 

Figure 2
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Fig. 2. Field testing (a) and schematic illustration (b) for cross hybridization between genetically modified (GM) and wild-type (WT) Zoysia grass according to a randomized complete block design. Black circle, GM Zoysia grass; white circle, WT Zoysia grass.

 

Figure 3
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Fig. 3. Field testing for gene flow from genetically modified (GM) to wild-type (WT) Zoysia grass within a 3-m radius. Each GM grass pot is surrounded by WT grass patches of 6 by 16 m2 area.

 

Figure 4
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Fig. 4. Field testing and schematic illustration for cross hybridization between genetically modified (GM) and wild-type (WT) Zoysia grass and it relative weed species as a function of distance. (a) GM Zoysia japonica tillers (illustrated with white circle). (b) WT grass group containing Zoysia japonica (Zj), Zoysia matrella (Zm), Zoysia sinica (Zs), Lolium perenne (Lp), and Poa pratensis (Pp). (c) Hexagonal arrangement illustrating a GM test area shown in (a); orange, 3-m radius; blue, 6-m radius; black, 9-m radius).

 

Figure 5
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Fig. 5. Test for the potential gene flow from genetically modified (GM) grass to wild-type (WT) grasses within a 3-km radius during a 2-yr period from 2003 to 2005. The GM grass field is centrally located in the Wimi-Ri test field in Nam Jeju County. The sampling sites shown were randomly chosen where Zoysia grasses grew. The sampling site distribution is biased in the north easterly direction from the GM grass site, whereas other directions are less favorable for grass growth due to geo-topographic factors (volcanic rocks, bushy jungles, forest, etc.).

 

Figure 6
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Fig. 6. Distance dependence for gene flow from the genetically modified (GM) to wild-type (WT) Zoysia grass within 9-m radius in field. The observed data can be best fit by an exponential equation resulting from a regression analysis of the data. Bars refer to standard error.

 

Figure 7
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Fig. 7. Zoysia japonica plants are overcame by dominant weed plants under natural ecological conditions. (A) Unmanaged Zoysia and weeds habitats. (B) The Zoysia lawn after weeds were removed. (C) Weeds began to overtake Zoysia grass (1 yr without weed control). (D), (E) The same as C after 2 and 3 yr without weed control, respectively.

 

Figure 8
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Fig. 8. Average wind velocity and directions on Jeju Island during the month of May 2005. The wind directions, maximum velocities of 6.7 m/s northerly; 5.6 m/s easterly and south easterly, 8.4 m/s southerly; 4 m/s south westerly, and 5.9 m/s westerly winds. Average monthly wind velocity was 5 m/s during the flowering season and gene flow testing.

 





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