HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Uyttebroek, M. Articles by Springael, D. Search for Related Content PubMed PubMed Citation Articles by Uyttebroek, M. Articles by Springael, D. GeoRef GeoRef Citation Agricola Articles by Uyttebroek, M. Articles by Springael, D. Related Collections Soil Microbiology Bioremediation and Biodegradation Soil Pollution

Differential Responses of Eubacterial, Mycobacterium, and Sphingomonas Communities in Polycyclic Aromatic Hydrocarbon (PAH)-Contaminated Soil to Artificially Induced Changes in PAH Profile

^a Division of Soil and Water Management, Catholic Univ. of Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium
^b Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, Avenida Reina Mercedes 10, Apartado 1052, E-41080 Seville, Spain
^c Bacteriology and Immunology, Veterinary and Agrochemical Research Centre (VAR), Groeselenberg 99, B-1180 Brussels, Belgium
^c Environmental and Process Technology, Flemish Institute for Technological Research (VITO), Boeretang 200, B-2400 Mol, Belgium

View larger version (15K): [in this window] [in a new window]   Fig. 1. Time lapse phenanthrene or pyrene concentrations in non-sterile or sterile soil K3663, spiked with phenanthrene (phe) or pyrene (pyr), respectively, during the K3663 incubation experiment. Error bars represent one standard error from six replicates.

View larger version (68K): [in this window] [in a new window]   Fig. 2. Fig. 3. 16S rRNA gene-based denaturing gradient gel electrophoresis community analysis of indigenous eubacteria (lanes 1–7) and indigenous Mycobacterium spp. (lanes 8–11) in soil for the K3663 soil experiment. Lanes 1–2: eubacterial fingerprints at day zero for the control soil and the acetone control soil, respectively. Lanes 3–7: eubacterial fingerprints for the non-sterilized soil spiked with phenanthrene after 0, 13, 29, 55, and 132 d of incubation, respectively. Lanes 8–11: Mycobacterium fingerprints after 13 d of incubation for the control soil, the acetone control soil and the non-sterilized soils spiked with phenanthrene and pyrene, respectively. Phe, phenanthrene; pyr, pyrene.

View larger version (18K): [in this window] [in a new window]   Fig. 3. Polycyclic aromatic hydrocarbon (PAH) concentration in the control soil and Tenax-extracted soil during the AndE soil incubation experiment. Error bars represent one standard error from four replicates. PAHs: phe, phenanthrene; pyr, pyrene; LMW, low-molecular-weight (PAHs with <4 rings of 16 EPA PAHs); HMW, high-molecular-weight (PAHs with >3 rings of 16 EPA PAHs).

View larger version (89K): [in this window] [in a new window]   Fig. 4. 16S rRNA gene-based denaturing gradient gel electrophoresis community analysis of indigenous eubacteria (lanes 1–10) and indigenous Sphingomonas spp. (lanes 11–18) in soil for the AndE soil experiment. Lanes 1, 3, 5, 7, 9: eubacterial fingerprints for control soil (C) after 0, 21, 35, 126, and 252 d of incubation, respectively. Lanes 2, 4, 6, 8, 10: eubacterial fingerprints for extracted soil (E) after 0, 21, 35, 126, and 252 d of incubation, respectively. Lanes 11–14: Sphingomonas fingerprints for control soil after 0, 21, 35, 126 d of incubation, respectively. Lanes 15–18: Sphingomonas fingerprints for extracted soil after 0, 21, 35, 126 d of incubation, respectively.