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Sediment Phosphorus Extractants for Phosphorus-31 Nuclear Magnetic Resonance Analysis

A Quantitative Evaluation

^a Dep. of Physical and Analytical Chemistry, Analytical Chemistry, Uppsala Univ., Box 599, 751 24 Uppsala, Sweden
^b Inst. of Biology, Univ. of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
^c Dep. of Biochemistry and Organic Chemistry, Organic Chemistry, Uppsala Univ., Box 599, 751 24 Uppsala, Sweden
^d Dep. of Ecology and Evolution, Limnology, Uppsala Univ., Box 573, 751 23 Uppsala, Sweden

View larger version (15K): [in this window] [in a new window]   Fig. 1. Typical spectra from the various extractants of the 0- to 2-cm layer of Lake Erken sediments.

View larger version (9K): [in this window] [in a new window]   Fig. 2. Interaction plot, with standard deviations, of the main extractants and their dependence on pre-extraction procedures (from the top to the bottom, EDTA, BD, and none) for the total extracted amount of P. BD, bicarbonate buffered dithionite.

View larger version (9K): [in this window] [in a new window]   Fig. 3. Principal components analysis (PCA) of the individual organic P groups extracted by the various extractants. In the PCA, the triplicate pre-extraction procedures are labeled: A = None, B = BD, C = EDTA, and the triplicates of the main extraction procedures: 1 = NaOH, 2 = NaOH-EDTA. The scoreplot (to the left) indicates how the main extractants vary from each other, and the loading plot (to the right) indicates on which organic P compounds this depends. BD, bicarbonate buffered dithionite.