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Identifying Fecal Sources in a Selected Catchment Reach Using Multiple Source-Tracking Tools

^a USGS, 5231 S. 19th St., Lincoln, NE 68512
^b USGS, 6480 Doubletree Ave., Columbus, OH 43229
^c Dep. of Environmental Engineering, Univ. of Cincinnati, Cincinnati, OH 45220
^d USEPA Office of Research and Development, National Risk Management Research Lab., 26 Martin Luther King Dr., MS-387, Cincinnati, OH 45268
^e Nebraska Dep. of Environmental Quality, 1200 "N" St., Suite 400, P.O. Box 98922, Lincoln, NE 68509

View larger version (34K): [in this window] [in a new window]   Fig. 1. Location of Plum Creek watershed in south-central Nebraska, including six sampling sites and contributing reach catchments on the main stem of Plum Creek.

View larger version (34K): [in this window] [in a new window]   Fig. 2. E. coli concentration in (A) water samples at the six sampling sites along Plum Creek and in (B) water and bed sediment samples at Site 2.

View larger version (26K): [in this window] [in a new window]   Fig. 3. Distribution of (A) isolates among sources and (B) operational taxonomic units (OTU) among sources in the known source library (inset pie chart in [B] depicts cosmopolitan OTUs).

View larger version (19K): [in this window] [in a new window]   Fig. 4. Pie charts depicting classification of (A) water-isolated E. coli and (B) sediment-isolated E. coli. Only isolates that could be assigned to a single host of origin are included in the plots.

View larger version (34K): [in this window] [in a new window]   Figure S1. Multi-dimensional scaling plot of isolates in the known source library. For effective discrimination by centroid-based statistical methods, the isolates should cluster by source.