JEQ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Published online 13 September 2006
Published in J Environ Qual 35:1873-1883 (2006)
DOI: 10.2134/jeq2005.0443
© 2006 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Abstract Freely available
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Astratinei, V.
Right arrow Articles by Lens, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Astratinei, V.
Right arrow Articles by Lens, P.
Agricola
Right arrow Articles by Astratinei, V.
Right arrow Articles by Lens, P.
Related Collections
Right arrow Industrial Wastes
Right arrow Toxic Trace Metals
Right arrow Microbial Processes
Right arrow Bioremediation and Biodegradation
Right arrow Other Environmental Contamination

Bioconversion of Selenate in Methanogenic Anaerobic Granular Sludge

Violeta Astratineib, Eric van Hullebuschc and Piet Lensa,*

a Sub-department of Environmental Technology, Wageningen University, P.O. Box 8129, EV Wageningen 6700, The Netherlands
b National Research and Development Institute for Environmental Protection, Splaiul Independentei 294, Bucharest 77703, Romania
c Laboratoire des Géomatériaux et Géologie de l'Ingénieur; Université de Marne la Vallée; Institut Francilien des Sciences Appliquées, Bât. IFI; 5, Boulevard Descartes-Champs sur Marne; 77454 Marne La Vallée, Cedex 2, France


Figure 1
View larger version (23K):

[in a new window]
  		Fig. 1. Effect of inoculum type on (a) selenate removal and (b) lactate consumption by sludge from waste water treatment plants; (c) selenate removal and (d) lactate consumption by natural inocula. (X) Umicore, ({blacktriangleup}) Eerbeek, ({blacksquare}) Veendam, ({diamondsuit}) Nedalco, ({square}) soil mining area, ({Delta}) river sediment, ({circ}) and sea sediment.

 

Figure 2
View larger version (25K):

[in a new window]
  		Fig. 2. Effect of electron donor (20 mM) on selenate removal by sludge Eerbeek incubated with 0.5 mM selenate. (a), (b) Selenate removal, and (c), (d) organic substrate consumption, with (•) propionate, (-) methanol, ({diamond}) acetate, ({blacksquare}) ethanol, ({blacktriangleup}) lactate, ({triangleup}) sucrose, ({square}) casein, ({circ}) glucose, (X) sterile reference and ({diamondsuit}) no substrate addition (endogenous substrate). Note that sucrose, glucose, and casein consumption was not determined.

 

Figure 3
View larger version (117K):

[in a new window]
  		Fig. 3. Elemental Se (Seo) in anaerobic granular sludge incubated with 0.5 mM selenate and (a) 20 mM lactate and (b) 20 mM ethanol (30°C; pH 7.5). (c) Red Seo and biomass in Eerbeek enrichment and (d) microorganisms in Eerbeek enrichment (mixed culture). Color change and formation of red Seo in the enrichment culture supplemented with (e) 10 mM selenate and 20 mM lactate (pH 7.5, 30°C).

 

Figure 4
View larger version (17K):

[in a new window]
  		Fig. 4. Spectra for red elemental Se collected (a) in the enrichment of Eerbeek sludge and (b) mineral precipitates in inoculum Eerbeek sludge incubated with 20 mM lactate, and respectively, 10 and 0.5 mM selenate. Unknown peaks did not match with the reference spectra of the achavalite, ferroselite, or other metal selenides (CaSe, CuSe, CoSe, NiSe, and ZnSe).

 

Figure 5
View larger version (14K):

[in a new window]
  		Fig. 5. Effect of selenate concentration on Se removal rate ({blacksquare}) and efficiency ({diamondsuit}) with (a) intact and (b) crushed Eerbeek sludge incubated with different concentrations of selenate and 20 mM lactate.

 

Figure 6
View larger version (16K):

[in a new window]
  		Fig. 6. Selenate removal from culture media in samples incubated with Eerbeek sludge and (a) 0.1 mM and (b) 0.5 mM selenate. ({diamondsuit}) sterile/inactive biomass and 20 mM lactate, ({blacksquare}) active biomass and 20 mM lactate, ({blacktriangleup}) and active biomass and no substrate addition (endogenous substrate).

 

Figure 7
View larger version (17K):

[in a new window]
  		Fig. 7. Removal of selenate by Eerbeek sludge incubated with 1 mM selenate and 20 mM lactate. ({diamondsuit}) Selenate, (•) selenite, and ({square}) total Se.

 

Figure 8
View larger version (17K):

[in a new window]
  		Fig. 8. Selenate (10 mM) reduction by Eerbeek enrichment with (•) 20 mM lactate or ({diamondsuit}) 20 mM ethanol as the electron donor. Closed symbols = soluble Se concentration; open symbols = optical density.

 




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Crop Science
Vadose Zone Journal Journal of Plant Registrations
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Copyright © 2006 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.