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Constitutive Expression of a High-Affinity Sulfate Transporter in Indian Mustard Affects Metal Tolerance and Accumulation

^a Biology Department, Colorado State University, Anatomy/Zoology Building, Fort Collins, CO 80523
^b Department of Molecular Biology, Sejong University, Kwangjin-Gu Kunja-Dong 98, Seoul 143-747, Korea
^c Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720

View larger version (80K): [in a new window]   Fig. 1. Transcript levels of S. hamata SHST1 in transgenic Indian mustard lines. Total RNA was isolated from 3-wk-old wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. (A) Ethidium bromide-stained gel showing total RNA loading. (B) Autoradiogram of RNA blot after hybridization with 33P-labeled SHST1 cDNA.

View larger version (44K): [in a new window]   Fig. 2. Seedling metal tolerance for wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. Shown is the ratio of seedling root length grown on medium containing metal(loid) relative to root length on control medium. The form and concentration used for each metal(loid) is described in Materials and Methods. Shown are means ± SE (n = 36). The letters above the bars indicate statistically significant differences between groups (ANOVA with pairwise post-hoc analyses, = 0.05).

View larger version (47K): [in a new window]   Fig. 3. Seedling metal accumulation for wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. Shown is the shoot metal concentration after 7 d of growth on agar medium supplied with a metal(loid) to a concentration as indicated in Materials and Methods. Shown are means ± SE (n = 5 samples pooled from 14 plants each). The letters above the bars indicate statistically significant differences between groups (ANOVA with pairwise post-hoc analyses, = 0.05).

View larger version (40K): [in a new window]   Fig. 4. Mature plant metal tolerance for wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. Shown is the ratio of plant fresh weight when grown on medium containing metal(loid) relative to its fresh weight on control medium. The form and concentration used for each metal(loid) is described in Materials and Methods. Shown are means ± SE (n = 10). The letters above the bars indicate statistically significant differences between groups (ANOVA with pairwise post-hoc analyses, = 0.05).

View larger version (39K): [in a new window]   Fig. 5. Mature plant shoot metal accumulation for wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. Shown is the shoot metal concentration after hydroponically grown plants were treated for 14 d with a metal(loid) concentration as indicated in Materials and Methods. Shown are means ± SE (n = 10). The letters above the bars indicate statistically significant differences between groups (ANOVA with pairwise post-hoc analyses, = 0.05).

View larger version (60K): [in a new window]   Fig. 6. Mature plant root metal accumulation for wild-type (WT) and SHST1 overexpressing (SHST1-4C and SHST1-12C) Indian mustard plants. Shown is the root metal concentration after hydroponically grown plants were treated for 14 d with a metal(loid) concentration as indicated in Materials and Methods. Shown are means ± SE (n = 5 samples pooled from two plants each). The letters above the bars indicate statistically significant differences between groups (ANOVA with pairwise post-hoc analyses, = 0.05)