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Release of Cryptosporidium and Giardia from Dairy Cattle Manure

Physical Factors

Jack F. Schijvena,*, Scott A. Bradfordb and Shihui Yangc

a National Institute of Public Health and the Environment, Microbiological Laboratory for Health Protection, P.O. Box 1, 3720 BA Bilthoven, the Netherlands
b George E. Brown, Jr. Salinity Laboratory, USDA-ARS, 450 West Big Springs Road, Riverside, CA 92507
c Microbiology Program, 241 Boyce Hall, University of California, Riverside, CA 92521



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Fig. 1. Experimental setup to elute (oo)cysts from a disk of manure.

 


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Fig. 2. Observed and simulated manure (a), oocyst (b), and cyst (c) release rates as a function of time at 5°C (Experiments 1 and 2) and 23°C (Experiments 3 and 4).

 


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Fig. 3. Observed and simulated manure (a), normalized oocyst (b), and normalized cyst (c) release rates as a function of time for Calf 1 (Experiment 1) and Calf 2 (Experiment 5) manure.

 


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Fig. 4. Observed and simulated manure (a), oocyst (b), and cyst (c) release rates as a function of time for manure composed of various cow manure mass fractions (fraction of cow manure, fcow, equals 0, 0.5, and 0.9 for Experiments 1, 6, and 7, respectively).

 


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Fig. 5. Observed and simulated manure (a), oocyst (b), and cyst (c) release rates as a function of time for drip (Experiment 1) and mist (Experiment 8) experiments. After 250 min the mist application was stopped for 41 h and switched to drip application for 50 min.

 


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Fig. 6. Observed and simulated manure (a), oocyst (b), and cyst (c) release rates as a function of time for high (10.5 mL min–1; Experiment 8) and low (2.9 mL min–1; Experiment 9) flow rates.

 





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