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Soil Microbial Communities Associated with Bt and Non-Bt Corn in Three Soils

Christopher B. Blackwood* and Jeffrey S. Buyer

USDA-ARS, Sustainable Agricultural Systems Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705



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  		Fig. 1. Canonical ordination of phospholipid fatty acid (PLFA) profiles derived from redundancy analysis of absolute peak areas. Percentages on axes are percent of total variation in dataset explained; 99% of the variability that can be attributed to experimental factors is shown by this plot. Symbols represent treatment means and bars are 95% confidence intervals for each treatment (corn line treatments have been pooled). Triangles, UM soil; squares, Salisbury soil; circles, Clarksville soil; white, Bt corn; black, non-Bt corn. PC, principal component.

 


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  		Fig. 2. Canonical ordination of bacterial community-level physiological profiles (CLPP) profiles derived from redundancy analysis. Plot details are as described for Fig. 1; 62% of the variability that can be attributed to experimental factors is shown by this plot. PC, principal component.

 


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  		Fig. 3. Canonical ordination of Clarksville soil community-level physiological profiles (CLPP) profiles derived from redundancy analysis. Percentages on axes are percent of total variation in dataset explained; 81% of the variability that can be attributed to experimental treatments is shown by this plot. Lines represent substrate vectors showing their contributions to the ordination. Only substrates with >22% of variance explained by the experimental treatments are shown. Each vector label corresponds to the well containing the substrate in GN Biolog plates; A8 = N-acetyl-D-glucosamine, A10 = L-arabinose, B6 = {alpha}-D-glucose, C1 = D-melibiose, C6 = D-sorbitol, E5 = {alpha}-keto valeric acid, F2 = succinamic acid, F9 = L-aspartic acid, G4 = L-ornithine, G6 = L-proline, H5 = phenyl ethylamine. Symbols represent positions of individual replicate profiles. Squares, Pioneer corn; circles, Syngenta corn; white, Bt corn; black, non-Bt corn. PC, principal component.

 




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