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Sequential Supercritical Fluid Extraction (SSFE) for Estimating the Availability of High Molecular Weight Polycyclic Aromatic Hydrocarbons in Historically Polluted Soils

Department of Environmental Biotechnology, Institute for Agrobiotechnology, Konrad Lorenz Strasse 20, 3430 Tulln, Austria

View larger version (77K): [in a new window]   Fig. 1. Relative extraction yields of four- to six-ring polycyclic aromatic hydrocarbons (PAHs) in the various extraction phases in percent of the total concentration (sum of all phases). Harsher extraction conditions are indicated by successively darker stacks (Phases I–V). The term * indicates that benzo(ghi)perylene was not detectable in soil TA1.

View larger version (17K): [in a new window]   Fig. 2. Relationship between molecular weight and decadic logarithm of relative Phase I extraction yields (readily desorbing polycyclic aromatic hydrocarbon [PAH] fraction) exemplarily shown for soils ES1 and AS3a.

View larger version (21K): [in a new window]   Fig. 3. Proportion of five- and six-ring polycyclic aromatic hydrocarbons (PAHs) in percent of the sum of all PAHs analyzed in this study. Error bars represent standard deviations (n = 3; for soils ES1 and BS1, n = 6).

View larger version (30K): [in a new window]   Fig. 4. Polycyclic aromatic hydrocarbon (PAHs) remaining in soil (in percent of an abiotic control) after 64 d of incubation with a PAH-degrading consortium. Error bars are standard errors of the mean (n = 3).

View larger version (19K): [in a new window]   Fig. 5. Polycyclic aromatic hydrocarbon (PAHs) remaining in soil after 64 d of biodegradation (in percent of an abiotic control) compared with PAHs remaining in soil after extraction under the second-mildest sequential supercritical fluid extraction (SSFE) condition (Phase I + II in percent of the total concentration). Circles represent individual PAHs.