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Targeted Sampling Protocol as Prelude to Bacterial Source Tracking with Enterococcus faecalis

^a Department of Crop and Soil Sciences, 3111 Plant Sciences, University of Georgia, Athens, GA 30602-7272
^b Marine Advisory and Technological Transfer Center, 715 Bay Street, Brunswick, GA 31520-4601

View larger version (67K): [in a new window]   Fig. 1. Location of the Sapelo River in Georgia.

View larger version (111K): [in a new window]   Fig. 2. Location of sample sites (numbers in boldface type) on the White Chimney and Sapelo Rivers showing numbers of fecal enterococci (numbers not in boldface type) from the (A) general and (B) targeted sampling on the headwaters of the Sapelo River. For the targeted sampling, the middle numbers represent sampling without local knowledge and the bottom numbers represent sampling with local knowledge. In the case of targeted sampling, only 8 of 11 samples without local knowledge and 11 of 51 samples with local knowledge are shown because the other sample locations are off of the map.

View larger version (18K): [in a new window]   Fig. 3. Dendrogram of restricted DNA ribotypes from 26 Enterococcus faecalis isolates obtained from humans and chickens. The restriction enzyme was PstI and the isolates are the same as those obtained by Wheeler et al. (2002).

View larger version (29K): [in a new window]   Fig. 4. Flow chart of targeted sampling. For purposes of brevity, portions of the flow chart for stormflow and marine or brackish water have been deleted, but are identical to the flow chart for freshwater.