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Enzymatic Oxidative Transformation of Chlorophenol Mixtures

^a Laboratory of Soil Biochemistry, Center for Bioremediation and Detoxification, 129 Land and Water Research Building, The Pennsylvania State University, University Park, PA 16802
^b Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente, Università di Napoli Federico II Via Università 100, 80055 Portici, Italy

View larger version (19K): [in a new window]   Fig. 1. Time course for transformation of 0.5 mM chlorinated phenols by 2 DMP units/mL laccase of Trametes villosa. Incubations were performed in phosphate buffer at pH 7 and 25°C (standard deviation was within 5%).

View larger version (22K): [in a new window]   Fig. 2. Time course for transformation of 4-chlorophenol (0.5 mM) by various concentrations of a laccase of Trametes villosa. Incubations were performed in phosphate buffer at pH 7 and 25°C (standard deviation was within 5%).

View larger version (26K): [in a new window]   Fig. 3. Transformation by 2 DMP units/mL laccase of Trametes villosa of 4-chlorophenol in the absence and presence of various concentrations of 2,4-dichlorophenol, and 2,4-dichlorophenol in the absence and presence of various concentrations of 4-chlorophenol. Incubations were performed at 25°C in phosphate buffer at pH 7 for 24 h (standard deviation was within 5%).

View larger version (60K): [in a new window]   Fig. 4. Transformation of various amounts of 4-chlorophenol by 2 DMP units/mL laccase of Trametes villosa in the absence and presence of 2,4-dichlorophenol and 2,4,6-trichlorophenol. Incubations were performed at 25°C in phosphate buffer at pH 7 for 24 h (standard deviation was within 5%).

View larger version (65K): [in a new window]   Fig. 5. Transformation of various amounts of 2,4-dichlorophenol by 2 DMP units/mL laccase of Trametes villosa in the absence and presence of 4-chlorophenol and 2,4,6-trichlorophenol. Incubations were performed at 25°C and pH 7 for 24 h (standard deviation was within 5%).

View larger version (68K): [in a new window]   Fig. 6. Transformation of various amounts of 2,4,6-trichlorophenol by 2 DMP units/mL laccase of Trametes villosa in the absence and presence of 4-chlorophenol and 2,4-dichlorophenol. Incubations were performed at 25°C and pH 7 for 24 h (standard deviation was within 5%).