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The Potential of Rhizosphere Microbes Isolated from a Constructed Wetland to Biomethylate Selenium

^a Research and Development Center, The Galilee Society (affiliated with Haifa University), P.O. Box 437, Shefa-Amr 20200, Israel
^b Institute of Phytospheric Research, Research Center Juelich, 52425 Juelich, Germany

View larger version (40K): [in a new window]   Fig. 1. Diagram of the aquatic microcosm vegetation facility (AMVF system) showing plant vegetation technique, six sampling points (Inlet, 1, 2, 3, 4, and Outlet), and Se supplementation device.

View larger version (25K): [in a new window]   Fig. 2. Effect of rhizosphere suspension (Rhiz) or cultured rhizosphere microbes (Cul) on total Se volatilized in 15 d. The 200-mL TSB cultivation solutions were supplemented with either 20 µM sodium selenate or sodium selenite. Each data point represents the mean value of three replicates. Mean values of total volatilized Se ± standard deviation are presented only in Day 15. The mean percentage of Se volatilized during 15 d of incubation is shown in the bar graph along with ± standard deviation. Different letters accompanying mean values of total Se volatilized (left side) and in the bar graph represent means with significant differences (Duncan multiple range test, p < 0.05).

View larger version (19K): [in a new window]   Fig. 3. Effect of rhizosphere suspension (Rhiz) or cultured rhizosphere microbes (Cul) on total Se volatilized in 15 d. The 300-mL half-strength Hoagland cultivation solutions were supplemented with either 20 µM sodium selenate or sodium selenite. Each data point represents the mean value of three replicates. Mean values of total volatilized Se ± standard deviation are presented only in Day 15. The mean percentage of Se volatilized during 15 d of incubation is shown in the bar graph along with ± standard deviation. Different letters accompanying mean values of total Se volatilized (left side) and in the bar graph represent means with significant differences (Duncan multiple range test, p < 0.05).