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Evaluation of Attachment of Cryptosporidium parvum and Giardia lamblia to Soil Particles

Xin Dai and Jan Boll*

Department of Biological and Agric. Eng., Univ. of Idaho, Moscow, ID 83844-2060



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  		Fig. 1. Example dot plot showing detection of C. parvum oocysts, G. lamblia cysts, and YG negative beads by the flow cytometer. Circles shown represent gates set for counting and sorting of particles as explained in the text.

 


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  		Fig. 2. Flow cytometer dot plots of AF positive beads with (a) YG negative beads, (b) ES oocysts, (c) PRL oocysts, (d) PHF oocysts, (e) G. lamblia cysts, and (f) YG negative beads with AF negative beads. Attached pairs were observed in a–e.

 


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  		Fig. 3. Confocal images of AF positive beads with (a) YG negative beads, (b) ES C. parvum oocysts, (c) PRL C. parvum oocysts, (d) PHF C. parvum oocysts, (e) G. lamblia cysts, and (f) PHF C. parvum oocysts with AF negative beads. Streaking is caused by particles moving during imaging.

 


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  		Fig. 4. Confocal image of PHF C. parvum oocyst in suspension with natural soil particles under transmitted light. Small circular objects are immersion oil drops added for viewing purposes.

 




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The SCI Journals Agronomy Journal Crop Science
Journal of Natural Resources
and Life Sciences Education		 Vadose Zone Journal
Soil Science Society of America Journal Journal of Plant Registrations The Plant Genome
Copyright © 2003 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.