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Optical Properties of Intact Leaves for Estimating Chlorophyll Concentration

Gregory A. Carter* and Bruce A. Spiering

Earth Science Applications Directorate, National Aeronautics and Space Administration, Stennis Space Center, MS 39529



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Fig. 1. Coefficient of determination (r2) versus wavelength ({lambda}) for relationships of leaf total chlorophyll (a + b) concentration with leaf reflectance (R), transmittance (T), and absorptance (A). Regressions were based on linear or quadratic models as indicated atop the figure and data combined among the four planar-leaved species (168 samples).

 


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Fig. 2. Coefficient of determination (r2) versus denominator wavelength ({lambda}) for relationships of leaf total chlorophyll (a + b) concentration with leaf reflectance (R), transmittance (T), and absorptance (A) band ratios. Regressions were based on linear or quadratic models as indicated atop the figure and data combined among the four planar-leaved species (168 samples). Ratios were computed by dividing R, T, or A at the best-fit {lambda} indicated in Fig. 1 by R, T, or A, respectively, at each {lambda} throughout the 400- to 850-nm spectrum. Where numerator and denominator {lambda} were identical, ratio values remained at unity regardless of chlorophyll concentration, yielding r2 = 0. These points were deleted from the figure to eliminate r2 = 0 spikes.

 


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Fig. 3. Coefficient of determination (r2) versus numerator wavelength ({lambda}) for relationships of leaf total chlorophyll (a + b) concentration with leaf reflectance (R), transmittance (T), and absorptance (A) band ratios. Regressions were based on linear or quadratic models as indicated atop the figure and data combined among the four planar-leaved species (168 samples). Ratios were computed by dividing R, T, or A at each {lambda} by R or T at 850 nm, or A at 400 nm based on results in Fig. 2.

 


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Fig. 4. Best-fit regressions of leaf total chlorophyll (a + b) concentration versus leaf reflectance (R), transmittance (T), absorptance (A), and band ratios. Regressions were based on a power function as indicated atop the figure and data combined among the four planar-leaved species. Regression parameters, including the coefficient of determination (r2) and standard deviation of the estimate (s), are listed for each regression (168 samples).

 


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Fig. 5. Best-fit regressions of leaf chlorophyll a and chlorophyll b concentrations versus leaf reflectance (R), transmittance (T), and absorptance (A) band ratios. Regressions were based on a power function as indicated atop the figure and data combined among the four planar-leaved species. Regression parameters, including the coefficient of determination (r2) and standard deviation of the estimate (s), are listed for each regression (168 samples).

 


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Fig. 6. Best-fit regressions in the far-red spectrum of leaf total chlorophyll (a + b) concentration versus leaf reflectance (R), transmittance (T), and absorptance (A) band ratios for each species. Regressions were based on the power function y = a + bxc. Regression parameters, including the coefficient of determination (r2) and standard deviation of the estimate (s), are listed for each regression along with the wavelength ({lambda}) for the numerator (42 samples per species).

 





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