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A Novel Technique for the Pre-Concentration and Extraction of Inositol Hexakisphosphate from Soil Extracts with Determination by Phosphorus-31 Nuclear Magnetic Resonance

Water Studies Centre and Chemistry Dep., Monash Univ., Clayton 3168, Victoria, Australia

View larger version (12K): [in a new window]   Fig. 1. (a) Axial form of phytic acid (pH 5–12) and (b) equatorial form of phytic acid (pH < 5 and pH > 12) (Martin and Evans, 1986). Pi = -OPO2-3, i = 1,2...6.

View larger version (29K): [in a new window]   Fig. 2. Phosphorus-31 nuclear magnetic resonance (NMR) spectra of a blank sample containing Sephadex G-25 (fine) gel and ultrapure water (top), a sample containing 1 mL of Sephadex gel and 40 mg P as phytic acid (center), and a sample of Sephadex gel that had been mixed with 10 mL of 0.5 M NaOH soil extract (bottom).