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A Novel Technique for the Pre-Concentration and Extraction of Inositol Hexakisphosphate from Soil Extracts with Determination by Phosphorus-31 Nuclear Magnetic Resonance

Benjamin L. Turner* and Ian D. McKelvie

Water Studies Centre and Chemistry Dep., Monash Univ., Clayton 3168, Victoria, Australia



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Fig. 1. (a) Axial form of phytic acid (pH 5–12) and (b) equatorial form of phytic acid (pH < 5 and pH > 12) (Martin and Evans, 1986). Pi = -OPO2-3, i = 1,2...6.

 


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Fig. 2. Phosphorus-31 nuclear magnetic resonance (NMR) spectra of a blank sample containing Sephadex G-25 (fine) gel and ultrapure water (top), a sample containing 1 mL of Sephadex gel and 40 mg P as phytic acid (center), and a sample of Sephadex gel that had been mixed with 10 mL of 0.5 M NaOH soil extract (bottom).

 





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The SCI Journals Agronomy Journal Crop Science
Journal of Natural Resources
and Life Sciences Education
Vadose Zone Journal
Soil Science Society of America Journal Journal of Plant Registrations The Plant Genome
Copyright © 2002 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.