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Phenanthrene Degradation in Soils Co-Inoculated with Phenanthrene-Degrading and Biosurfactant-Producing Bacteria

^a Dep. of Plant and Soil Sciences, Univ. of Delaware, Newark, DE 19717
^b Dep. of Civil and Environmental Engineering, Univ. of Delaware, Newark, DE 19717

View larger version (24K): [in a new window]   Fig. 1. Phenanthrene mineralization in aqueous cultures containing P. strain R, isolate P5-2, or co-culture of these strains with the surfactant-producing bacterium, P. aeruginosa ATCC 9027. Data points are means of determinations from triplicate experimental flasks and vertical bars represent one standard deviation about the mean.

View larger version (17K): [in a new window]   Fig. 2. Phenanthrene degradation rates plotted against initial phenanthrene (PHE) concentration in aqueous culture. Data points are means of determinations from triplicate experimental cultures and vertical bars represent one standard deviation about the mean.

View larger version (25K): [in a new window]   Fig. 3. Phenanthrene mineralization in co-inoculated Kenansville (A) and Fallsington (B) soil. Data were normalized by P. strain R or Isolate P5-2 biomass (388 or 210 mg, respectively) and are presented as means of determinations from triplicate experimental microcosms. Vertical bars correspond to one standard deviation about the mean.

View larger version (26K): [in a new window]   Fig. 4. Effect of 5 x critical micelle concentration (CMC) biosurfactant amendment on phenanthrene (PHE) mineralization in co-inoculated Kenansville (A) and Fallsington (B) soil. Data were normalized by P. strain R or Isolate P5-2 biomass (388 or 210 µg, respectively) and are presented as means of determinations from triplicate experimental microcosms. Vertical bars represent one standard deviation about the mean.

View larger version (20K): [in a new window]   Fig. 5. Effect of rhamnolipid addition on phenanthrene (PHE) dissolution kinetics in Kenansville (A) and Fallsington (B) soil. Data points are means of determinations from triplicate microcosms and vertical bars represent one standard deviation about the mean.