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TECHNICAL REPORTS

Heavy Metals in the Environment

Extraction of Lead and Cadmium from Soils by Cysteine and Glutathione

^a Dep. of Biological and Environmental Engineering, Cornell Univ., Riley-Robb Hall, Ithaca, NY 14853
^b Dep. of Civil and Environmental Engineering, Univ. of Maryland, Baltimore County, 1000 Hilltop Cir., TRC 181, Baltimore, MD 21250

^* Corresponding author (baa7{at}cornell.edu).

Received for publication December 17, 2008. Metal-thiol complexes can enhance metal uptake by plant roots and microorganisms, therefore it is important to determine whether thiols effectively solubilize these metals from the soil matrix. Extractions were conducted by combining 1 g contaminated soil and 10 mL of 10 mmol L^–1 thiol solution and shaking for 1 h. Both cysteine and glutathione extracted between 5 and 45% of Pb and Cd from laboratory and field-contaminated soils at pHs > 6 after 1 h. In the presence of oxygen, the half-life of reduced cysteine was on the order of 0.1 h and dissolved metal concentrations decreased to nearly zero over 24 h. In extractions with glutathione, both the metals and thiol were more stable, with a half-life for glutathione of 23 h, and stable dissolved metal concentrations over 24 h in the presence of oxygen. In cysteine extractions, Pb was primarily removed from the Fe/Mn oxide fraction of the amended soil and dissolved Fe concentrations followed dissolved Pb concentrations, whereas this pool of Pb was unavailable to thiol extraction in aged field contaminated soils. Iron is hypothesized to play a role in the oxidation of cysteine via both reductive dissolution of iron oxides and in the photolytic oxidation of the Fe-thiol complex in the aqueous phase. While overall cysteine was more effective than glutathione at extracting Pb from soils, its propensity to oxidize may limit its ability to increase the bioavailability of this metal to plants or microorganisms in oxic environments.

Abbreviations: DTPA, Diethylenetriaminepentaacetic acid • EDTA, Ethylenediaminetetratacetic acid • HEPES, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid • MES, 2-(N-Morpholino) ethanesulfonic Acid • PBS, phosphate buffered saline