HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by He, Z. Articles by Sims, J. T. Search for Related Content PubMed PubMed Citation Articles by He, Z. Articles by Sims, J. T. Agricola Articles by He, Z. Articles by Sims, J. T. Related Collections Phosphorus Experiment Design Animal Waste

TECHNICAL REPORTS

Waste Management

Comparison of Phosphorus Forms in Wet and Dried Animal Manures by Solution Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy and Enzymatic Hydrolysis

^a USDA-ARS, New England Plant, Soil, and Water Lab., Orono, ME 04469
^b Geological and Environmental Sciences, Stanford Univ., Stanford, CA 94305
^c Biological and Agricultural Engineering, Univ. of Arkansas, Fayetteville, AR 72701
^d Dep. of Plant and Soil Sciences, Univ. of Delaware, Newark, DE 19716

^* Corresponding author (Zhongqi.He{at}ars.usda.gov)

Received for publication December 19, 2006. Both enzymatic hydrolysis and solution ³¹P nuclear magnetic resonance (NMR) spectroscopy have been used to characterize P compounds in animal manures. In this study, we comparatively investigated P forms in 0.25 M NaOH/0.05 M EDTA extracts of dairy and poultry manures by the two methods. For the dairy manure, enzymatic hydrolysis revealed that the majority of extracted P was inorganic P (56%), with 10% phytate-like P, 9% simple monoester P, 6% polynucleotide-like P, and 18% non-hydrolyzable P. Similar results were obtained by NMR spectroscopy, which showed that inorganic P was the major P fraction (64–73%), followed by 6% phytic acid, 14 to 22% other monoesters, and 7% phosphodiesters. In the poultry manure, enzymatic hydrolysis showed that inorganic P was the largest fraction (71%), followed by 15% phytate-like P and 1% other monoesters, and 3% polynucleotide-like P. NMR spectroscopy revealed that orthophosphate was 51 to 63% of extracted P, phytic acid 24 to 33%, other phosphomonoesters 6 to 12%, and phospholipids and DNA 2% each. Drying process increased orthophosphate (8.4% of total P) in dairy manure, but decreased orthophosphate (13.3% of total P) in poultry manure, suggesting that drying treatment caused the hydrolysis of some organic P to orthophosphate in dairy manure, but less recovery of orthophosphate in poultry manure. Comparison of these data indicates that the distribution patterns of major P forms in animal manure determined by the two methods were similar. Researchers can utilize the method that best fits their specific research goals or use both methods to obtain a full spectrum of manure P characterization.

Abbreviations: NMR, nuclear magnetic resonance

This article has been cited by other articles:

				Z. He, C. W. Honeycutt, T. S. Griffin, B. J. Cade-Menun, P. J. Pellechia, and Z. Dou Phosphorus Forms in Conventional and Organic Dairy Manure Identified by Solution and Solid State P-31 NMR Spectroscopy J. Environ. Qual., July 23, 2009; 38(5): 1909 - 1918. [Abstract] [Full Text] [PDF]

				U. P. P. Pillai, V. Manoharan, A. Lisle, X. Li, and W. Bryden Phytase Supplemented Poultry Diets Affect Soluble Phosphorus and Nitrogen in Manure and Manure-amended Soil J. Environ. Qual., June 23, 2009; 38(4): 1700 - 1708. [Abstract] [Full Text] [PDF]

				Z. Dou, C. F. Ramberg, J. D. Toth, Y. Wang, A. N. Sharpley, S. E. Boyd, C. R. Chen, D. Williams, and Z. H. Xu Phosphorus Speciation and Sorption-Desorption Characteristics in Heavily Manured Soils Soil Sci. Soc. Am. J., January 21, 2009; 73(1): 93 - 101. [Abstract] [Full Text] [PDF]

				R. Zhang, F. Wu, Z. He, J. Zheng, B. Song, and L. Jin Phosphorus Composition in Sediments from Seven Different Trophic Lakes, China: A Phosphorus-31 NMR Study J. Environ. Qual., January 13, 2009; 38(1): 353 - 359. [Abstract] [Full Text] [PDF]

				Z. He, C. W. Honeycutt, B. J. Cade-Menun, Z. N. Senwo, and I. A. Tazisong Phosphorus in Poultry Litter and Soil: Enzymatic and Nuclear Magnetic Resonance Characterization Soil Sci. Soc. Am. J., August 20, 2008; 72(5): 1425 - 1433. [Abstract] [Full Text] [PDF]

				K. Gungor, A. Jurgensen, and K. G. Karthikeyan Determination of Phosphorus Speciation in Dairy Manure using XRD and XANES Spectroscopy J. Environ. Qual., October 24, 2007; 36(6): 1856 - 1863. [Abstract] [Full Text] [PDF]