HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Related articles in JEQ Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Schroder, J. L. Articles by Si, J. Search for Related Content PubMed PubMed Citation Articles by Schroder, J. L. Articles by Si, J. GeoRef GeoRef Citation Agricola Articles by Schroder, J. L. Articles by Si, J. Related Collections Toxic Trace Metals Remediation Heavy Metals Soil Pollution Soil Chemistry

TECHNICAL REPORT

Heavy Metals in the Environment

Validation of the In Vitro Gastrointestinal (IVG) Method to Estimate Relative Bioavailable Lead in Contaminated Soils

^a Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078
^b School of Natural Resources, The Ohio State University, Columbus, OH 43210
^c Veterinary Medicine Diagnostic Laboratory, University of Missouri-Columbia, Columbia, MO 65205
^d Department of Statistics, Oklahoma State University, Stillwater, OK 74078

^* Corresponding author (basta.4{at}osu.edu).

Received for publication February 2, 2003. The effect of the dosing vehicle (e.g., dough) on the ability of an in vitro gastrointestinal (IVG) method to predict relative bioavailable Pb associated with soil ingestion was evaluated. Bioaccessible Pb determined by the IVG method was compared with relative bioavailable Pb measured from dosing trials using juvenile swine for 18 contaminated soils ranging from 1270 to 14200 mg Pb kg^–1. Bioaccessible Pb was measured in the IVG gastric extraction (GE) and intestinal extraction (IE) solutions. Mean bioaccessible Pb values were 32.2% for GE without dough, 23.0% for GE with dough, 1.06% for IE without dough, and 0.56% for IE with dough. It is possible that phytic acid associated with the dough addition decreased bioaccessible Pb. In vivo relative bioavailable Pb ranges for different swine tissues were 1 to 87% for blood, 0 to 110% for liver, 1 to 124% for kidney, and 0.04 to 94% for bone. Strong linear relationships between IVG GE Pb with dough (r > 0.76, P < 0.0002), IVG IE Pb with dough (r > 0.56, P < 0.015), and IVG GE Pb without dough (r > 0.81, P < 0.0001) and in vivo bioavailable Pb as estimated with blood, kidney, liver, and bone were found. Inexpensive in vitro methods may be useful in providing an estimate of the variability in relative bioavailable Pb at a single study site. The IVG method can be used to estimate relative bioavailable Pb, As, and Cd in contaminated soil.

Abbreviations: GE, gastric extraction • IE, intestinal extraction • IVG, in vitro gastrointestinal • PBET, physiologically based extraction test • SRM, standard reference material

Related articles in JEQ:

This Issue in Journal of Environmental Quality

JEQ 2004 33: 413-418. [Full Text]  

This article has been cited by other articles:

				D. G. Beak, N. T. Basta, K. G. Scheckel, and S. J. Traina Bioaccessibility of Lead Sequestered to Corundum and Ferrihydrite in a Simulated Gastrointestinal System J. Environ. Qual., October 27, 2006; 35(6): 2075 - 2083. [Abstract] [Full Text] [PDF]

				O. Furman, D. G. Strawn, G. H. Heinz, and B. Williams Risk Assessment Test for Lead Bioaccessibility to Waterfowl in Mine-Impacted Soils J. Environ. Qual., February 2, 2006; 35(2): 450 - 458. [Abstract] [Full Text] [PDF]

				G. S. Plumlee, S. A. Morman, and T. L. Ziegler The Toxicological Geochemistry of Earth Materials: An Overview of Processes and the Interdisciplinary Methods Used to Understand Them Reviews in Mineralogy and Geochemistry, January 1, 2006; 64(1): 5 - 57. [Full Text] [PDF]