HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Related articles in JEQ Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by You, Y. Articles by Jin, Y. Search for Related Content PubMed PubMed Citation Articles by You, Y. Articles by Jin, Y. GeoRef GeoRef Citation Agricola Articles by You, Y. Articles by Jin, Y. Related Collections Water Quality Batch Studies Laboratory Column Studies Remediation

TECHNICAL REPORTS

Ground Water Quality

Sorption of MS2 Bacteriophage to Layered Double Hydroxides

Effects of Reaction Time, pH, and Competing Anions

^a Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19717-1303
^b Department of Renewable Resources, University of Wyoming, Laramie, WY 82071-3354

^* Corresponding author (yjin{at}udel.edu).

Received for publication February 19, 2003. Batch sorption and column breakthrough studies were conducted to investigate the potential of layered double hydroxides (LDHs) to remove bacteriophage MS2 from contaminated waters. All four of the LDHs evaluated in this study had very high retention capacities for MS2. Sorption results showed that MS2 could be completely removed from 5.2 x 10² plaque-forming units (pfu)/mL solution by Mg–Al LDH 2 (i.e., 2:1 Mg to Al ratio LDH), with the highest sorption capacity observed in this study of 1.51 x 10¹⁰ pfu/g. Attachment of MS2 to LDHs was a rapid process and reached quasi-equilibrium after a 1-h reaction time. Within the pH range studied (pH 4–9), Mg–Al LDH 2 showed high sorption potential for MS2 at all pH values but sorption decreased slightly with increasing solution pH. Background solution anions influenced virus sorption, with SO^2-₄ and HPO^2-₄ decreasing sorption significantly whereas the presence of NO^-₃ had little effect on the attachment of MS2 to Mg–Al LDH 2. The addition of another virus (X174) only caused a slight decrease in the retention of MS2 by Mg–Al LDH 2, suggesting that there was insignificant competitive sorption between MS2 and X174 on LDH surfaces. Results from column experiments indicate that there was no MS2 breakthrough from columns packed with Mg–Al LDH 2–coated sand, suggesting complete MS2 retention at the virus concentration tested. The high mass recovery by beef extract solution revealed that the removal of viruses by the LDH was due to sorption of MS2 to LDH surfaces, rather than inactivation.

Abbreviations: AGW, artificial ground water • LDH, layered double hydroxide • pfu, plaque-forming units • SEM, scanning electron microscope • TAEC, theoretical anion exchange capacity

Related articles in JEQ:

This Issue in Journal of Environmental Quality

JEQ 2003 32: 1931-1938. [Full Text]  

This article has been cited by other articles:

				J. Langlet, F. Gaboriaud, C. Gantzer, and J. F. L. Duval Impact of Chemical and Structural Anisotropy on the Electrophoretic Mobility of Spherical Soft Multilayer Particles: The Case of Bacteriophage MS2 Biophys. J., April 15, 2008; 94(8): 3293 - 3312. [Abstract] [Full Text] [PDF]

				S. A. Bradford, Y. F. Tadassa, and Y. Jin Transport of Coliphage in the Presence and Absence of Manure Suspension J. Environ. Qual., August 9, 2006; 35(5): 1692 - 1701. [Abstract] [Full Text] [PDF]

				K. P. O'Connell, J. R. Bucher, P. E. Anderson, C. J. Cao, A. S. Khan, M. V. Gostomski, and J. J. Valdes Real-Time Fluorogenic Reverse Transcription-PCR Assays for Detection of Bacteriophage MS2 Appl. Envir. Microbiol., January 1, 2006; 72(1): 478 - 483. [Abstract] [Full Text] [PDF]