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A Novel Technique for the Pre-Concentration and Extraction of Inositol Hexakisphosphate from Soil Extracts with Determination by Phosphorus-31 Nuclear Magnetic Resonance

Water Studies Centre and Chemistry Dep., Monash Univ., Clayton 3168, Victoria, Australia

* Corresponding author (bturner{at}nwisrl.ars.usda.gov)

Received for publication June 2, 2000. Inositol hexakisphosphate (IP₆) is often the dominant form of soil organic phosphorus (P), but is rarely investigated because of the analytical difficulties encountered in its extraction, separation, and detection in environmental samples. In particular, recent advances in the study of soil organic P with ³¹P nuclear magnetic resonance (NMR) have been of limited use for the study of IP₆, because the technique does not discriminate between IP₆ and other forms of P. This was addressed by developing a novel analytical procedure using the retentive properties of gel-filtration gels for IP₆, which allows the combined selective extraction and pre-concentration of IP₆ from soil extracts with determination by ³¹P NMR. While the technique is still in the developmental stage, the results demonstrate that the gel does not interfere with ³¹P NMR analysis and retains IP₆ to concentrations well above those required to give clear spectral signals. The technique has considerable potential for application to the study of IP₆ in soil extracts and water samples and, with development, could help to answer fundamental questions regarding the dynamics of organic P in the environment.

Abbreviations: IP₆, inositol hexakisphosphate • ³¹P NMR, phosphorus-31 nuclear magnetic resonance

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