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Oxidation and Acidification of Anaerobic Sediment-Water Systems by Autoclaving

Dep. of Environ. Science and Eng., Oregon Graduate Inst. of Science and Technol., 19600 N.W. von Neumann Dr., Beaverton, OR 97006-1999;

N. Lee Wolfe

Environ. Res. Lab., USEPA, Athens, GA 30613-7799.

^* Corresponding author.

ABSTRACT

The effect of autoclaving on the acid/base and redox properties of anaerobic sediment slurries was determined to facilitate the interpretation of autoclaved controls in studies of environmental reduction reactions. Autoclaving decreased electrode measurements of pH by 0.26 to 0.68 units and increased electrode measurements of Eh by 34 to 94 mV. To corroborate this effect, we added redox indicators to the slurries and observed that autoclaving caused a shift in color consistent with the change in electrode measurements. Further investigation demonstrated that the observed effects are a common characteristic of autoclaving anaerobic sediment and are not limited to very restricted sediment types and are not artifacts of the way the experiments were performed.

Mention of trade names or commercial product does not constitute endorsement or recommendation by the USEPA.

Received for publication May 26, 1992.

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