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Acetylene-Reduction Assay of Anaerobic Nitrogen Fixation by Sediments of Selected Wisconsin Lakes¹

ABSTRACT

Sediments from two hardwater and six softwater lakes of varying trophic levels from Wisconsin were incubated under conditions which permitted continued synthesis of methane. Rates of acetylene reduction indicated that, on the average, sediments from hardwater and softwater lakes may fix 37 and 7.7 ng of N, respectively, per gram (dry weight) per day. The rate of acetylene reduction diminished in the presence of nitrogenase inhibitors.

Key Words: eutrophication

¹ Research supported by College of Agricultural and Life Sciences, Univ. of Wisconsin, Madison, and by a grant from U. S. Environmental Protection Agency, No. R-801362. Presented on Nov. 2, 1972 before Div. A-5, Soil Sci. Soc. Amer. at the annual meeting held in Miami Beach, Florida.

² Post Doctoral Fellow and Associate Professor, respectively, Department of Soil Science, Univ. of Wisconsin, Madison 53706. The senior author is currently Senior Lecturer, Department of Soil Science, Massey Univ., Palmerston North, New Zealand.

Received for publication October 25, 1972.