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Suitability of Freezing as a Method of Preserving Runoff Samples for Analysis of Soluble Phosphate¹

ABSTRACT

Slow freezing of surface runoff samples decreased the levels of soluble orthophosphate in the water phase from 2 to 27%, but low temperature storage at 2C for 3 days or freezing of the water phase after sediment removal did not alter the orthophosphate concentrations of samples. Rapid freezing of runoff samples in liquid nitrogen resulted in decreases in orthophosphate content which were about one-half of those observed for conventional freezing. Frozen storage is not a good method for preserving runoff samples to be analyzed for soluble phosphate unless sediment is removed prior to freezing.

Key Words: water analysis • phosphorus methodology • ice formation

¹ Contribution from the Department of Agronomy, Purdue Univ., and the Corn Belt Branch, Soil & Water Cons. Res. Div., ARS-USDA, Lafayette, Ind. 47907. Published as Journal Paper No. 4508 of the Indiana Agr. Exp. Sta.

² Assistant Professor of Agronomy and Soil Scientist (Physics), SWC, respectively.

Received for publication October 13, 1971.

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