Fig. 2. Full spectrum (A) and expanded central region (B) of the heteronuclear multiple quantum coherence (HMQC) spectrum acquired at 600 MHz using 128 scans. Region 1 is consistent with C–H bonds from various aliphatic structures including fatty acids and amino acids side chains. Region 2 is consistent with units in amino acids, sugars, methoxy, and methylene groups next to ester and ether groups. Region 3 is consistent with aromatic fragments (Simpson et al., 2001).